P-006 In men with Raised Sperm DNA Fragmentation Index, would advance Sperm Preparation techniques alter Embryo Euploidy?

Abstract Study question In men with Raised Sperm DNA Fragmentation Index (DFI), Would advance sperm preparation techniques at Intra-Cytoplasmic Injection (ICSI) alter embryo euploidy status? Summary answer raised DFI, use of selection are safe and do not appear to ploidy status the embryos What is known already MACS (Magnetic Activated Cell Sorting), Microfluidic Sorting (MF), surgically retrieved Testicular (TESA) Double Density Gradient processing (DDG) have been shown optimize ICSI for DFI. any these methods proven design, size, duration This was a retrospective observational study conducted private fertility centre in-between years 2015 & 2022. Couples where women (<37yrs) had idiopathic Recurrent Implantation Failures (RIF) DFI (>25% DFI) underwent Pre-Implantation Genetic Testing (PGT) were evaluated (n = 473). Based on method population divided into four groups. TESA 84), 105), MF(n 135), DDG 148).A total 1802 in this study. Participants/materials, setting, All Controlled Ovarian Stimulation (COS) oocyte retrieval male partners as per clinics SOP. Appropriate counselling written consents obtained from all couples, applicable. oocytes subjected ICSI, extended blastocyst culture trophoectoderm (TE) biopsy. Post biopsy vitrified TE tissue Next-Generation Sequencing (NGS) assess status. Ploidy between various compared. Main results role chance Euploidy rates groups follows: 311 screened)- 48% 445 screened) – 51% MF 489 61% 557 54% Though numerically highest group euploid embryos, there seems be no statistical significance (p-value 0.973). Different seem Ejaculated or sperms similar Use sperm, which considered immature doesn’t increase aneuploidy. MACS, seemed embryos. further warrants research validate routine intervention reproductive outcomes. Limitations, reasons caution Retrospective un-equal size small sample well-designed needed findings Wider implications Role implantation yet proven. Data showed alarming aneuploidy younger comparable published literature. Trial registration number applicable